Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 4;11:1178. doi: 10.1038/s41467-020-14981-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Genotyping the T1 progeny of T0-48A. The purpose of each PCR experiment and the genotyping primers used are shown to the left for each gel panel. a Primers Cas9p-Genotyping-F and nos-Terminator-R amplify a 534 bp DNA fragment in plants with the Cas9 module. b Primers M13F and 1R amplify a 1.8 kb DNA fragment in plants with the off-target insertion of the pAcc-B donor plasmid. c Primers 1F and 2F amplify a 2.3 kb DNA fragment in plants with the carotenoid cassette inserted at Target B. d Primers 1F and 3R amplify a 1.9 kb genomic DNA fragment in plants unless the carotenoid cassette at Target B is homozygous. The positions of the primers used are illustrated in Figs. 1a, b, 2a. Kitaake (K) was used as the negative control. The red triangle at the bottom marks 48A-7, which is free of the co-integrated CRISPR and donor plasmids. Source data are provided as a Source Data file.