Table 3.
Central composite design matrix, representing the response of NAP and NAR activities as influenced by significant factors along with the predicted activity, residuals and concentrations of variables levels.
| Run Order | NAP- CCD matrix | Actual NAP Activity (U/mL) | Predicted NAP Activity (U/mL) | St. Residual | Actual NAR Activity (U/mL) | Predicted NAR Activity (U/mL) | St. Residual | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (X1) pH | (X2) FeCl2.4H2O | (X3) Inoculum Size % | (X4) Na2MoO4. 2H2O | (X5) Sodium citrate | ||||||||
| NAR- CCD matrix | ||||||||||||
| (X1) pH | (X2) FeCl2.4H2O | (X3) Na2MoO4. 2H2O | (X4) Sodium citrate | (X5) NaNO3 | ||||||||
| 1 | 0 | 2 | 0 | 0 | 0 | 1059.29 | 1063.131 | −0.18 | 1421.0 | 1396.8 | 0.65 | |
| 2 | −1 | 1 | −1 | −1 | −1 | 519.29 | 537.477 | −1.52 | 819.3 | 855.8 | −1.78 | |
| 3 | −1 | −1 | −1 | −1 | 1 | 428.57 | 445.102 | −1.39 | 830.7 | 830.2 | 0.02 | |
| 4 | 1 | 1 | 1 | −1 | −1 | 1022.14 | 1022.416 | −0.02 | 748.3 | 728.5 | 0.96 | |
| 5 | 1 | 1 | −1 | 1 | −1 | 1282.14 | 1263.797 | 1.54 | 386.6 | 400.4 | −0.67 | |
| 6 | −1 | 1 | −1 | 1 | 1 | 717.29 | 714.763 | 0.21 | 1666.0 | 1690.1 | −1.17 | |
| 7 | 0 | −2 | 0 | 0 | 0 | 525 | 522.478 | 0.12 | 1338.0 | 1393.5 | −1.49 | |
| 8 | 0 | 0 | 0 | 2 | 0 | 894.29 | 929.885 | −1.64 | 276.0 | 282.4 | −0.17 | |
| 9 | 1 | −1 | −1 | 1 | 1 | 532.86 | 512.862 | 1.68 | 469.0 | 445.8 | 1.13 | |
| 10 | 0 | 0 | 0 | 0 | 0 | 602.86 | 560.743 | 1.34 | 406.7 | 371.1 | 0.66 | |
| 11 | −1 | −1 | 1 | 1 | 1 | 879.29 | 879.506 | −0.02 | 1835.2 | 1810.4 | 1.21 | |
| 12 | 0 | 0 | −2 | 0 | 0 | 463.57 | 466.533 | −0.14 | 558.2 | 515.9 | 1.13 | |
| 13 | 0 | 0 | 0 | 0 | 0 | 533.57 | 560.743 | −0.86 | 306.7 | 371.1 | −1.19 | |
| 14 | 0 | 0 | 0 | −2 | 0 | 756.43 | 722.155 | 1.58 | 133.2 | 158.1 | −0.67 | |
| 15 | −1 | −1 | 1 | −1 | −1 | 889.86 | 910.789 | −1.75 | 1698.8 | 1686.4 | 0.6 | |
| 16 | 1 | 1 | −1 | −1 | 1 | 1100.29 | 1096.168 | 0.35 | 998.5 | 990.6 | 0.38 | |
| 17 | 0 | 0 | 2 | 0 | 0 | 996.57 | 994.926 | 0.08 | 487.1 | 560.8 | −1.97 | |
| 18 | −2 | 0 | 0 | 0 | 0 | 724.29 | 686.825 | 1.73 | 1595.0 | 1556.0 | 1.05 | |
| 19 | 1 | −1 | 1 | 1 | −1 | 917.23 | 901.629 | 1.31 | 515.2 | 480.1 | 1.71 | |
| 20 | −1 | 1 | 1 | 1 | −1 | 1117.29 | 1119.161 | −0.16 | 594.0 | 606.1 | −0.59 | |
| 21 | 2 | 0 | 0 | 0 | 0 | 1035.43 | 1074.215 | −1.79 | 393.4 | 463.7 | −1.88 | |
| 22 | 0 | 0 | 0 | 0 | 2 | 739.29 | 746.441 | −0.33 | 309.3 | 359.1 | −1.34 | |
| 23 | −1 | −1 | −1 | 1 | −1 | 602.86 | 605.17 | −0.19 | 387.0 | 408.3 | −1.04 | |
| 24 | 0 | 0 | 0 | 0 | 0 | 558.23 | 560.743 | −0.08 | 400.0 | 371.1 | 0.53 | |
| 25 | 1 | −1 | 1 | −1 | 1 | 897.14 | 895.761 | 0.12 | 100.1 | 43.3 | 2.77 | |
| 26 | 0 | 0 | 0 | 0 | 0 | 561.6 | 560.743 | 0.03 | 389.9 | 371.1 | 0.35 | |
| 27 | 0 | 0 | 0 | 0 | −2 | 816.43 | 810.598 | 0.27 | 105.0 | 86.4 | 0.5 | |
| 28 | 1 | −1 | −1 | −1 | −1 | 662.86 | 663.575 | −0.06 | 330.0 | 319.3 | 0.52 | |
| 29 | 0 | 0 | 0 | 0 | 0 | 522.14 | 560.743 | −1.23 | 361.9 | 371.1 | −0.17 | |
| 30 | 1 | 1 | 1 | 1 | 1 | 1334.71 | 1314.273 | 1.71 | 470.0 | 437.7 | 1.57 | |
| 31 | −1 | 1 | 1 | −1 | 1 | 892.86 | 908.953 | −1.35 | 337.2 | 327.6 | 0.47 | |
| 32 | 0 | 0 | 0 | 0 | 0 | 587.38 | 560.743 | 0.85 | 393.0 | 371.1 | 0.4 | |
| Variable | Coded levels/Experimental Values | |||||||||||
| −2 | −1 | 0 | 1 | 2 | ||||||||
| pH | 5.8 | 6.3 | 6.8 | 7.3 | 7.8 | |||||||
| Inoculum Size (0.5 McFarland) (%) | 2.5 | 4 | 5 | 7.5 | 10 | |||||||
| NaNO3 (g/L) | 4 | 5.5 | 7 | 8.5 | 10 | |||||||
| FeCl2.4H2O (mg/L) | 300 | 350 | 400 | 450 | 500 | |||||||
| Na2MoO4.2H2O (mg/L) | 30 | 70 | 100 | 150 | 200 | |||||||
| Sodium citrate (g/L) | 4 | 5.5 | 7 | 8.5 | 10 | |||||||