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. 2020 Mar 4;10:4021. doi: 10.1038/s41598-020-60843-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of peripheral blood T cell responses to AFP-derived epitopes. The number of spots on IFN-γ ELISPOT is shown. The response was determined to be positive when >10 specific spots were calculated and there were more than twice the number of spots in wells with peptides than in those without peptides. The amino acid sequence of each peptide is shown in Table 2. *indicates >50 spots.