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. 2020 Mar 4;10:4021. doi: 10.1038/s41598-020-60843-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Responses of CD8⁺ and CD4⁺ cell-depleted PBMCs to AFP-derived epitopes. The numbers of spots on IFN-γ ELISPOT and the HLA-DR alleles of each patient are shown. Black and white bars denote the results for CD8⁺ and CD4⁺ cell-depleted PBMCs, respectively. The positive rate of CD8⁺ cell-depleted PBMCs against AFP₁ was 35.7% (5/14), while that of CD4⁺ cell-depleted PBMCs was 14.3% (2/14). ND denotes “not determined”. *indicates >30 spots.