Table 4.

Univariate and multivariate Cox proportional-hazards regression analysis for overall survival. Abbreviations: OS: Overall survival; HR: Hazard Ratio; CI: Confidence Interval; p: p-value; CTCs: Circulating tumour cells; miR: miRNA; B: Baseline; 12w: 12 weeks; 24w: 24weeks; *p < 0.05.

			Univariate analysis			Multivariate analysis
Characteristics		Median OS (months)	HR	95% CI	p	HR	95% CI	p
All patients		27
Gender	Female	23.5	1.04	0.52–2.09	0.918	1.35	0.60–3.04	0.467
	Male	27
Age (years)	>55	28	0.93	0.46–1.89	0.842	0.55	0.22–1.41	0.213
	<55	22
Primary tumour location	Colon	27	0.95	0.49–1.84	0.883
	Rectum	27.5
Metastasis location	Liver	29			0.328
	Lung	26	1.48	0.67–3.26	0.334
	Other	18.5	2.07	0.74–5.78	0.167
Metachronous metastasis	Yes	27	1.22	0.47–3.17	0.684
	No	27.5
Metastasis surgery	Yes	45	0.55	0.25–1.23	0.146
	No	27
K-RAS status	Mutated	30	0.73	0.36–1.47	0.374
	Wild-Type	27
CEA (B)	High	25	1.31	0.61–2.82	0.490
	Standard	29
Ca 19.9 (B)	High	26	1.07	0.54–2.11	0.848
	Standard	29
CEA (12w)	High	24.5	2.34	1.04–5.26	0.041*
	Standard	37
Ca 19.9 (12w)	High	24.5	1.58	0.76–3.29	0.222
	Standard	35
CEA (24w)	High	22	2.85	1.25–6.51	0.013*
	Standard	37
Ca 19.9 (24w)	High	26	1.62	0.75–3.45	0.220
	Standard	35
Response (12w)	Favourable	30.5	0.38	0.18–0.78	0.008*
	Non-favourable	15
Response (24w)	Favourable	36	0.356	0.17–0.76	0.007*	0.32	0.13–0.81	0.017*
	Non-favourable	18
Number of CTCs (B)			1.12	0.98–1.28	0.089
Number of CTCs (12w)			1.04	1.00–1.07	0.045*
Number of CTCs (24w)			1.13	0.90–1.42	0.285
miR-222 (24w)	High	25	2.55	1.16–5.58	0.02*	2.65	1.15–6.13	0.023*
	Low	34
miR-92 (24w)	High	27	1.63	0.81–3.32	0.174
	Low	30