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. 2020 Mar 4;10:4064. doi: 10.1038/s41598-020-61053-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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T-cell profiling in TETs. (a) Clustering analysis for the T-cell profiling of TETs (n = 31). The T-cell parameters of CD4 and CD8 single-positive T cells measured by flow cytometry are listed. Clinical information shows the pathological WHO histological classification and Masaoka stage. The SUVmax (n = 24) of FDG-PET/CT (b) and tumor volumes (c) were compared between hot and cold clusters. Tumor volumes were calculated by the following formula: tumor volume (cm³) = length × width × thickness/2. Measurements were performed using surgically resected specimens. Means with SD were shown. (d) Disease-free survival curve of patients in the hot and cold clusters.