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. 2020 Mar 4;10:4064. doi: 10.1038/s41598-020-61053-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of each immune checkpoint molecule on CD4 and CD8 single-positive T cells in B3/C and non-B3/C TET tissues. The percentages of PD-1 (a), Tim-3 (b), CD103 (c), 4-1BB (d), and OX-40 (e) expression on CD8 or CD4 single-positive T cells were analyzed by flow cytometry. The ratio of CD4+ CD25++ in CD4 single-positive T cells was also indicated (f). Means with SD were shown.