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. 2020 Mar 4;10:4064. doi: 10.1038/s41598-020-61053-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytokine production by CD8 and CD4 single-positive T cells in B3/C and non-B3/C TETs with the PMA/ionomycin stimulation. (a) Freshly isolated cells from 14 TETs were stimulated with PMA and ionomycin, and the production of IFN-γ, TNF-α, and IL-2 in CD8 single-positive T cells was analyzed by intracellular cytokine staining. Means with SD were shown. (b) Purified CD4 single-positive T cells by a cell sorter from eight TETs were stimulated with PMA and ionomycin, and the production of IFN-γ, TNF-α, and IL-2 was analyzed by intracellular cytokine staining. Means with SD were shown.