Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 4;10:4040. doi: 10.1038/s41598-020-61051-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HSD17B12 KD induces alterations in cellular lipid metabolism. Huh7.5 parental cells transduced with lentiviruses expressing shNT (NT) or shHSD17B12 (HSD17B12) were used to determine the relative cellular abundance of (A) triglycerides (TG), (B) palmitic acid (C16:0), (C) oleic acid (C18:1), (D) oleic/palmitic (C18/C16) ratio, (E) phosphatidylethanolamine (PE) and (F) phosphatidylcholine (PC) among all detected lipid species by shotgun lipidomics analysis of whole cell extracts. The data of three experiments were pooled for the analysis (n = 3). Data were normalized to shNT control arbitrary value of 1 (except D). Non-parametric Mann Whitney test was used to compare each test condition with the control. P values < 0.05 (*) or non-significant (NS) are indicated in comparison with cells expressing shNT as control.