Table 1. Statistics of 3D reconstruction and model refinement.
Data collection | |
Electron microscope | Titan Krios |
Electron detector | Falcon III |
Voltage (kV) | 300 |
Defocus range (µm) | 0.5–2.1 |
Pixel size (Å) | 0.833 |
Electron dose (e− Å−2) | 32 |
Images | 792 |
3D reconstruction | |
Final particles | 15320 |
Applied symmetry | D3 |
Resolution (Å) | 3.1 |
B factor (Å2) | −72 |
Model composition | |
Peptide chains | 2 |
Residues | 3780 |
Cofactors | FMN, NADPH |
Ramachandran plot | |
Favored (%) | 94.27 |
Outliers (%) | 0.13 |
Validation | |
MolProbity score | 1.96 |
Rotamer favored (%) | 94.01 |
Rotamer outliers (%) | 1.49 |