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. 2020 Feb 25;147(4):dev181693. doi: 10.1242/dev.181693

Fig. 3.

Fig. 3.

Induction of meiosis is compromised in the cortical germ cells of D17 embryos subject to oestrogen level alterations. (A) D17 (HH43) left gonad sections immunostained for γH2AX (green) and P63 (red); nuclei are counterstained with DAPI (blue). (B) Sections from the D17 (HH43) left gonad shown in A immunostained for γH2AX (green) and SYCP3 (red). ZW-WT, ZW wild type; ZW-Fa, ZW treated with fadrozole from D7-7.5 (HH31); ZW-E2, ZW treated with β-oestradiol at D7-7.5; ZZ-E2, ZZ treated with β-oestradiol at D7-7.5; ZW-Fa(sr), ZW, partially sex-reversed gonad, treated with fadrozole at D4; ZZ-E2(sr), ZZ, partially sex-reversed gonad, treated with β-oestradiol at D4. All gonadal models have a cortical domain containing germ cells. In ZW-Fa ovary and ZW-E2 ovary, most cortical germ cells express SYCP3 like the ZW-WT control. In ZW-Fa(sr) ovotestis, very few germ cells express γH2AX and SYCP3 (orange dotted circled areas). In ZZ-E2(sr) ovotestis and ZZ-E2 testis overlain by a cortex, some cortical germ cells express γH2AX but none expresses SYCP3. White dotted line highlights the cortical domain borders. See Fig. S4 for the medullary structure of the ZZ-E2, ZW-Fa(sr) and ZZ-E2(sr) models.