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. 2019 Jul 18;133(5):jcs230508. doi: 10.1242/jcs.230508

Fig. 6.

Fig. 6.

VASP knockdown does not perturb EVL stability or localization in NKL cells. NKL cells were nucleofected with one of two siRNAs targeting VASP (siVASP #1 or #2) or a control siRNA (siCtrl). (A) A sample of these cells from each experiment were lysed and evaluated by immunoblotting for the indicated proteins. (B,C) NKL cells were allowed to adhere to mIgG-coated latex beads (B) or anti-NKG2D/2B4-coated latex beads (C) for 30 min, fixed and evaluated by immunofluorescence for the localization of EVL. Representative images are shown. (D) Quantification of three independent experiments for each group. The red dotted line indicates the average value for all mouse IgG bead conjugates across the three-siRNA groups. Confocal scale bars indicate 10 µm unless otherwise indicated. Images represent three independent experiments. Dot graph shows all data points from three independent experiments. Error bars indicate s.e.m. from the indicated mean. ***P<0.0005 (Student's t-test).