Fig. 8.

Model depicting the recruitment of EVL complexes following NKG2D–DAP10 signaling. Binding of NKG2D to its ligand induces Lck-dependent tyrosine (Y85) phosphorylation of the YINM motif within the transmembrane signaling adaptor protein DAP10. Tyrosine phosphorylation leads to the recruitment of the Grb2–VAV1 complex, which facilitates the recruitment of EVL complexes containing VASP, WASP and probably DOCK8, leading to F-actin generation at the CS. F-actin at the synapse drives cell adhesion to target cells, formation of the cytotoxic synapse and, ultimately, NK cell cytotoxicity.