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. 2020 Feb 26;11:169. doi: 10.3389/fimmu.2020.00169

Table 1.

The primer sequences used in the present study.

Species Gene name Sequences References
Monkey HPRT1 F:5′-TGACACTGGCAAAACAATGCA-3′ (30)
R:5′-GGTCCTTTTCACCAGCAAGCT-3′
IL-1β F:5′-GCGGCAACGAGGATGACTT-3′ (32)
R:5′-TGGCTACAACAACTGACACGG-3′
IL-8 F:5′-GGAACCATCTCGCTCTGTGTAA-3′ (32)
R:5′-GGTCCACTCTCAATCACTCTCAG-3′
TNF-α F:5′-CACCACGCTCTTCTGTCT-3′ (32)
R:5′-AGATGATCTGACTGCCTGAG-3′
MCP-1 F:5′-CTTCTGTGCCTGCTGCTCATA-3′ (32)
R:5′-ACTTGCTGCTGGTGATTCTTCT-3′
Pig RPL4 F:5′-GGAAACCGTCGCGAGA-3′ (28)
R:5′-GCCCCAGAGACAGTT-3′
GBP-2 F:5′-ACCAGGAGGTTTTCGTCTCTCTATT-3′ Present study
R:5′-TCCTCTGCCTGTATCCCCTTT-3′
IFIT3 F:5′-GCATTTTCCAGCCAGCATC-3′ (33)
R:5′-TCTGTTCCTTTCCTTTCCTTCCT-3′
OAS1 F:5′-TGGTGGTGGAGACACACACA-3′ (33)
R:5′-CCAACCAgAgACCCATCCA-3′
IFN-α F:5′-ACTCCATCCTGGCTGTGAGGAAAT-3′ (33)
R:5′-ACTCCATCCTGGCTGTGAGGAAAT-3′
IFN-β F:5′-ATGTCAGAAGCTCCTGGGACAGTT-3′ (34)
R:5′-AGGTCATCCATCTGCCCATCAAGT-3′
PEDV PEDV-M F:5′-TCCCGTTGATGAGGTGAT-3′ Present study
R:5′-AGGATGCTGAAAGCGAAAA-3′
PEDV-N F:5′-CGCAAAGACTGAACCCACTAACTT-3′ (35)
R:5′-TTGCCTCTGTTGTTACTCGGGGAT-3′

Abbreviations: HPRT1, hypoxanthine phosphoribosyltransferase 1; RPL4, ribosomal protein L4; IL-1β, interleukin-1β; IL-8, interleukin-8; TNF-α, tumor necrosis factor α; MCP-1, monocyte chemotactic protein 1; PEDV-M, membrane protein (porcine epidemic diarrhea virus); PEDV-N, nucleocapsid protein (porcine epidemic diarrhea virus); GBP-2, guanylate-binding proteins; IFIT3, interferon-induced protein 3; OAS1, 2′-5′oligoadenylatesynthetase 1; IFN-α, interferon-α; IFN-β, interferon-β.