(A) Timeline of combined developmental and functional imaging of spinal cord development.
(B) Maximum-intensity projections of whole-embryo developmental imaging data at four time points. Colored spheres show tracked precursors of spinal neurons that are active by 22 hpf. Cell-type specific marker (mnx) not shown. Time stamps (hrs:min:sec): imaging time.
(C) Dorsal view of reconstructed tracks of all active neurons in spinal circuit. Colors: time (left) or random colors for lineages (right).
(D) Lineage tree and activity profiles of all active neurons for data shown in (C). Horizontal order in tree determined by medio-lateral cell position in neural plate. For neurons involved in patterned activity, fraction of activity variance explained by shared factors is shown below tree. Grey dots at end of branches mark siblings that are not active neurons.
Scale bars: 100 μm (B).
See Movie S1; Fig. S7.