Figure 2.

Patient‐derived GICs efficiently captured SLNPs via CXCR4‐stimulated macropinocytosis. a) SDF1 mimic peptides (FH27/FH29/FH38) modification enhanced the cellular uptake of DiI‐labeled SLNPs. SLNPs were prepared at the peptides: DMPC, molar ratio 1:100, and incubated with GICs at 37 °C for 3.5 h at the DMPC concentration of 20 µg mL⁻¹ (n = 3). The significance of the differences was evaluated by one‐way ANOVA followed by Bonferroni test (*p < 0.05, ****p < 0.0001). b) Cellular uptake of DiI‐SLNPs and colocalization of SLNPs to CXCR4. Scale bar, 100 µm. c) The FH38‐DiI‐SLNPs showed the highest percentage of colocalization with CXCR4. d) Knockdown of CXCR4 led to a reduction in the cellular uptake of DiI‐LNPs and DiI‐SLNPs (n = 3). The significance of the differences between two groups (**p < 0.01, ****p < 0.0001) was evaluated by two‐tailed Student's t‐test. e) Qualitative analysis of the GICs uptake of LNPs and SLNPs after knocking down CXCR4. Scale bar, 100 µm. f) Colocalization of DiI‐LNPs and DiI‐SLNPs to macropinocytosis marker FITC‐70 kDa dextran in GICs in the absence/presence of EIPA (150 × 10⁻⁶ m). Scale bar, 100 µm.