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. 2020 Jan 20;7(5):1903290. doi: 10.1002/advs.201903290

Figure 4.

Figure 4

miR34a‐SLNPs inhibited the self‐renewal and reversed temozolomide resistance of GICs. a,b) The protein level of SOX2 in GICs after treatment with NC‐LNPs, NC‐SLNPs, miR34a‐LNPs, and miR34a‐SLNPs at the miRNA concentration 100 × 10−9 m for 12 h. c) Laser confocal imaging of the SOX2 protein expression in GICs after treatment with miR34a‐LNPs and miR34a‐SLNPs at the miRNA concentration 100 × 10−9 m for 24 h. Scale bar, 50 µm. d) Representative images and e) quantitative analysis of in vitro colony formation assay of GICs treated with miR34a‐LNPs and FH38‐miR34a‐SLNPs for 48 h (n = 3). The significance of the differences between two groups (*p < 0.05, ***p < 0.001) was evaluated by two‐tailed Student's t‐test. f,g) The protein level of Notch1 in GICs after treatment with NC‐LNPs, NC‐SLNPs, miR34a‐LNPs, and miR34a‐SLNPs at the miRNA concentration 100 × 10−9 m for 24 h. h) Cell viability of GICs after treatment with NC‐LNPs, miR34a‐LNPs, and miR34a‐SLNPs at the concentration of 50 × 10−9 m miR34a combination with the different concentration of TMZ (5 × 10−6, 20 × 10−6, 50 × 10−6, 100 × 10−6, and 200 × 10−6 m) for 24 h. For (b) and (g), data represent mean ± s.d. (n = 3). The significance of the differences was evaluated by one‐way ANOVA followed by Bonferroni test (*p < 0.05, ***p < 0.001, ****p < 0.0001).