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. 2020 Jan 20;7(5):1903290. doi: 10.1002/advs.201903290

Figure 6.

Figure 6

Safety of miR34a‐LNPs and FH38‐MiR34a‐SLNPs on healthy ICR mice. a) Cell viability of the primary astrocytes after the treatment with miR34a‐LNPs, FH27‐miR34a‐SLNPs, FH29‐miR34a‐SLNPs, and FH38‐miR34a‐SLNPs. b–d) Healthy ICR mice were intravenously treated with saline miR34a‐LNPs or FH38‐miR34a‐SLNPs at the siRNA dose of 0.36 mg kg−1 every 3 d for totally five doses (n = 4). Blood biochemistry and hematology tests were performed at 1 d after the last injection. AFU: alpha‐L‐fucosidase, ALB: albumin, ALP: alkaline phosphatase, ALT: alanine aminotransferase, AMY: amylase, AST: aspartate transaminase, CK: creatine kinase, DBIL: direct bilirubin, GLB: globulin, GLU: glucose, GPDA: glycyl proline dipeptidyI aminopeptidase, IBIL: indirect bilirubin, LDH: lactate dehydrogenase, TBA: thiobarbituric acid, TBIL: total bilirubin, TC: total cholesterol, TG: triglyceride, TP: total protein, CR: creatinine, UREA: urease, UA: uric acid, BASO: basophils, EO: eosinophils, HB: hemoglobin, HCT: hematocrit, LYMPH: lymphocytes, MCH: mean corpuscular hemoglobin, MCHC: mean corpuscular hemoglobin concentration, MCV: erythrocyte mean corpuscular volume, MONO: monocytes, MPV: mean platelet volume, NEUT: neutrophils, PCT: procalcitonin, PDW: platelet distribution width, PLT: platelet, RBC: red blood cell, RDW‐CV: red cell distribution width (coefficient of variation), WBC: white blood cell. Data represent mean ± s.d. Unpaired student's t‐test (two tailed) was used for comparison between two groups and p < 0.05 were considered significant. e) Hematoxylin and eosin staining of the major organs. Scale bar, 50 µm.