Figure 1.

Rhinovirus C15 (RV-C15) significantly increases airway reactivity to carbachol (Cch)-induced bronchoconstriction. Human precision cut lung slices were stimulated with RV-C15 (10⁵–10⁷ pfu) or diluent for 48 hours. Constriction of small airways within each slice was assessed to a Cch dose response (10⁻⁸–10⁻⁴ M). (A–C) Dose–response curves (A), log of the effective concentration to induce 50% bronchoconstriction (Log EC₅₀) values (B), and area under the curve (C) were plotted and compared. Data represent n = 5 separate donors with at least three slices/treatment for each donor (mean ± SEM). *P < 0.05. Two-tailed unpaired t tests comparing each condition with control buffer, as well as a two-way ANOVA, were performed. pfu = plaque-forming unit.