Figure 4.

RV-C15 augments Cch- but not histamine (Hist)-induced intracellular calcium flux. Human airway epithelial cells (HAEC) were exposed to RV-C15 (10⁶ pfu; 48 h). (A–F) Conditioned medium was transferred to serum-starved human airway smooth muscle (HASM), and intracellular calcium flux (Fluo-8 dye) was measured after (A–C; green) Cch (10 μM) or (D–F; purple) Hist (1 μM) stimulation. (A–F) Time courses after agonist stimulation (A and D), peak calcium response (B and E), and area under the curve (C and F) are shown. Data represent mean ± SEM of four separate donors. *P < 0.05 compared with control buffer. Two-tailed paired t tests comparing each condition with control buffer were performed, as was two-way ANOVA. Rfu = relative fluorescence units.