Figure 6.

Candidate antagonists of LL-37. (A) Phosphatidylinositol (PI), cardiolipin (CL), phosphoethanolamine (PE), and phosphocholine (PC) were subjected to the same TLC and LL-37 binding IB conditions as shown in Figure 5. (B) Comparison of the migration of M. chimaera 12-54-Sw-B-1 and 12-7-S-1-3 total lipid extracts (TLEs) with pure PI and CL by TLC and LL-37 binding IB. (C) E. coli was added to LL-37 (25 μg/ml) incubated with PI, CL, PE, or PC or M. chimaera 12-54-Sw-B-1 and 12-7-S-1-3 TLEs or vehicle controls. Loss of killing of E. coli was used as a readout for LL-37 inactivation.