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Deletion of PAI-1 in ATII cells blocked TGF-β1–induced secretion of profibrotic mediators. ATII cells isolated from WT and PAI-1−/− mice were treated with TGF-β1, and the CM was collected at Day 3 after TGF-β1 treatment as described in Figure 1. (A–D) The amounts of IL-4, IL-6, IL-13, and PDGF in the CM were measured by ELISA (n = 3–6).
