Figure 7.

Inhibition of PAI-1 activity attenuated the stimulatory effect of L2 cell SASP on macrophage gene expression. Rat macrophage NR8383 cells were incubated with the CM from L2 cells treated with saline or TGF-β1 in the presence or absence of TM5275 as described in Figure 5 for 24 hours. The expression levels of mRNAs were assessed by quantitative real-time PCR as described in the Methods section, and the results were expressed as fold changes relative to NR8383 cells incubated with the CM from saline- and solvent-treated L2 cells (n = 3–4). P < 0.05. α = significantly different from NR8383 cells incubated with the CM from saline- and solvent-treated L2 cells; β = significantly different from NR8383 cells incubated with the CM from TGF-β1– plus solvent-treated L2 cells; nd = not detected.