Diabetes-associated retinal vascular changes in mice with disrupted rod
phototransduction. A, Representative photomicrograph of retinal vascular tree
isolated from STZ-treated wild type mice by trypsin digest of neuroglial
elements followed by PAS and hematoxylin staining. B and C, Representative
high-power micrographs showing atrophic capillaries in STZ-treated wild type
mice, denoted by arrowheads. Each series of three arrowheads was scored as one
individual atrophic capillary. D, Quantification of capillary lesions in
STZ-treated mice. Data are represented as median (line), IQR (box), and range
(whiskers); n=>3 mice/group. **** = P<0.0001 by 2-way ANOVA with
Bonferroni post-hoc correction.