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. 2020 Mar 4;15(3):e0229712. doi: 10.1371/journal.pone.0229712

Fig 1. Overview of CRISPR/Cas9 genome editing.

Fig 1

A. Sequence of human EGFR exon 21 for CRISPR/Cas9 genome editing. sgRNA target sites and protospacer adjacent motifs are indicated by red bars and blue characters, respectively. The ssODN sequence as a repair template is also shown. Box represents Leu or Arg residue at amino acid position 858. B. Timeline of genome editing. Cas9n (sgRNA) plasmids and ssODN for homology directed recombination (HDR) are transfected into A549 cells. Successful transfection and HDR were validated by the presence of GFP-positive cells at day 1 and a mutation-specific PCR product at day 4, respectively. Transfected cells were clonally expanded to isolate L858R-knockin clones.