Fig 5. Addition of 2-DG decreases cellular plasma membrane order.
A.) (Left) Cartoon diagram depicting a model of the planarizable, push-pull membrane order probe FlipTR, constructed from two large dithienothiophene flippers, in Ld and Lo environments. (Right) Representative image of FlipTR-stained cells in untreated conditions depicting the homogenous distribution of lifetimes throughout the entirety of the cell; scale bar 25μm. B.) (Top) Representative images of FlipTR-stained cells in listed treatment conditions depicting the extracted τm; scale bar 25μm. (Right) Bar charts depicting the average long lifetime (τ1) in TZM-bl cells under listed treatment conditions (n = at least 30 per condition, collected during the course of three independent experiments). * p<0.05 ** p<0.01 *** p<0.001 as determined by one-way ANOVA.