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. 2020 Feb 21;16(2):e1008359. doi: 10.1371/journal.ppat.1008359

Fig 6. Acute treatment of TZM-bl cells with 2-DG increases plasma membrane tension.

Fig 6

A.) (Left) Cartoon diagram model depicting membrane tension FRET probe MSS and its control probe (insensitive to membrane tension), KMSS. (Right) Representative pseudocoloured FRET efficiency (i.e. Ypet/eCFP) images of TZM-bl cells expressing MSS under listed treatment conditions; scale bar 10μm. B.) (Left) Dot plots depicting donor CFP lifetimes of the tension-sensitive MSS probes extracted from single cells in the treatment conditions listed (n = at least 20/condition, collected during the course of three independent experiments; *p<0.5, **p<0.01 *** p<0.001 as determined by either one-way ANOVA). (Right) Dot plots depicting donor CFP lifetimes of the tension-insensitive KMSS probes extracted from single cells in the treatment conditions listed (n = at least 30/condition, collected during the course of three independent experiments; *p<0.5, **p<0.01 *** p<0.001 as determined by either one-way ANOVA).