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Crystallization and glycan soaking conditions for S. Typhi PltB homopentamer.
| PDB ID | Protein | Conc. (mg/ml) |
Crystallization condition | Glycan | Soaking condition | Cryo-protection |
|---|---|---|---|---|---|---|
| 6TYN | PltB | 5.1 |
26% PEG1500, Sodium acetate pH 4.4 |
Neu5,9Acs α2–3 Gal β1–4 GlcNAc | 5 mM, 30 min, RT | 20% Ethylene glycol |
| 6TYO | PltB | 5.1 | Neu4,5Acs α2–3 Gal β1–4 GlcNAc | 5 mM, 30 min, RT | 20% Ethylene glycol | |
| 6TYQ | PltB | 5.1 | Neu5,9Acs α2–6 Gal β1–4 GlcNAc | 3 mM, 2hr, RT | 20% Ethylene glycol |
