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. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: Sci Transl Med. 2020 Jan 22;12(527):eaax1501. doi: 10.1126/scitranslmed.aax1501

Figure 5: Tau and microtubule-stabilizing compounds impair wild-type EGFR stability and signaling in gliomas.

Figure 5:

a–d, WB analysis and quantification of phosphorylated EGFR (pEGFR) in SVZ EGFRwt/amp (a), RG1 (b), SVZ EGFR vIII (c) and 12o150 (d) tumors after the overexpression of GFP or Tau. Actin was used as a loading control. e, WB analysis of total EGFR and Acetylated tubulin (Acetyl-tub) in SVZ EGFR wt/amp and RG1 tumors after the overexpression of GFP or Tau. Acting was used as a loading control. f, Kaplan-Meier overall survival curves of mice that were orthotopically injected with RG1 cells and subsequently treated with intraperitoneal injections (2/week) of EpoD (1 mg/Kg) (n=8). g,h WB analysis and quantification of pEGFR, total EGFR and Acetyl-tub in the tumors (f). Actin was used as loading control. i, Kaplan-Meier overall survival curves of mice that were orthotopically injected with RG1 cells and subsequently treated with intraperitoneal injections (2/week) of EpoD (1mg/Kg) and/or TMZ (5mg/Kg/day) (n=6). *, P ≤0.05, **, P ≤0.01, ***, P ≤0.001. n.s. non significant.