Figure 4.

Differential pressure regulation of Ca²⁺ spark frequency in high fat–fed and low fat–fed arteries. A, Confocal image of an intact pressurized mesenteric artery from a low-fat diet mouse with representative F/F_o traces (B and C) derived from regions of interest (ROI) as shown on the image at 20 mmHg (B) and 80 mmHg (C) of intraluminal pressure. D, Confocal image of artery from high-fat diet mice with changes in F/F_o at ROI at both 20 mmHg (E) and 80 mmHg (F) intraluminal pressure (for quantification of Ca²⁺ spark characteristics, see Figure S6). F/F_o indicates fluorescence intensities relative to baseline.