Figure 4.
Effect of miR-346 inhibition or activation on TNF-α and VDR expression in cell lines. Caco-2 cells were untransfected (control) or transfected with miR-346 inhibitor (a, b) or miRNA-346 mimic (c). Twenty-four hours after transfection, levels of miR-346, TNF-α, and VDR transcript were quantified by qRT-PCR. Suppression of miR-346 by miR-346 antisense molecules was confirmed in both cell lines. Inhibition of miR-346 led to the strong upregulation of TNFα and VDR in both HepG2 (a) and Caco-2 (b) cell lines. Induction of miR-346 led to a significant downregulation of TNFα, VDR, and p27kip1 in Caco-2 cells (c). Each experiment was repeated 4 times with similar results. Data are presented as mean ± SE. TNF-α, tumor necrosis factor-α; VDR, receptor of vitamin D.