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. 2020 Mar 4;9:e51406. doi: 10.7554/eLife.51406

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© 2020, Siems et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) K-means clustering was performed for the mRNA profiles of those 1046 proteins in our myelin proteome inventory for which significant mRNA expression was found by RNA-Seq in the sciatic nerve of rats dissected at ages E21, P6, P18 and 6 months (M6). Note that this filtering strategy allows to selectively display the developmental abundance profiles of those transcripts that encode myelin-associated proteins rather than of all transcripts present in the nerve. Standardized mRNA abundance profiles are shown (n = 4 biological replicates per age). Known myelin genes are displayed in red. For comparison, Pmp22 mRNA was included although the small tetraspan protein PMP22 was not mass spectrometrically identified due to its unfavorable distribution of tryptic cleavage sites. Normalized counts for all mRNAs including those displaying developmentally unchanged abundance are provided in Figure 3—source data 1. (B) Numbers of mRNAs per cluster.

Figure 3—source data 1. Normalized developmental mRNA abundance data → sheet 1: normalized values for all individual 4 biological replicates per age → sheet 2: normalized values for biological replicates averaged to give mean per age.

elife-51406-fig3-data1.xlsx^{(399.2KB, xlsx)}