Table 1. Residues in close proximity to the positions corresponding to ScCdc3 Thr302 or His262 for other septins with solved crystal structures.
| PDB entry, Septin•nucleotide (oligomeric state) |
Residues within 5 Å (capitalized) of position corresponding to ScCdc3 Thr302* |
Residues within 5 Å (capitalized) of His corresponding to ScCdc3 His262 |
|---|---|---|
| 2QNR, HsSEPT2•GDP (G dimer) |
trans loop 1 (pfgHGlkp) | α4 (reRLkkRildE) |
| 2QA5, HsSEPT2•GDP (G dimer) |
trans loop 1 (pfgHglkp) | α4 (rerLkkRilde) |
| 3FTQ, HsSEPT2•GppNHp (G dimer) |
trans loop 1 (pfgHGlkp) | α4 (rerLkkRildE), Switch I (kieRtvq)†, Switch II (dtpgygDain)† |
| 4Z51, HsSEPT3•GppNHp (monomer) | trans loop 1 (ptGHSlrp) | α4 (kseFkqRvrkE) |
| 3SOP, HsSEPT3•GDP (G dimer) |
trans loop 1 (ptGHSlrp) | α4 (kseFkqRvrke),, P-loop (gqSGlgk)†, Switch I (vIPKtve)† |
| 4Z54, HsSEPT3•GDP (G dimer) |
trans loop 1 (ptGHSlrp) | α4 (kseFkqRvrkE), P-loop (gqSGlgk)†, Switch I (eeKIPKtve)† |
| 3T5D, HsSEPT7•GDP (G dimer) |
trans loop 1 (psgHGlkp) | α4 (qqFkkQim), P-loop (geSGlgk)† |
| 3TW4, HsSEPT7•GDP (G dimer) |
trans loop 1 (psgHGLkp) | α4 (qqFkkQim), P-loop (geSGlgk)† |
| 5CYO, HsSEPT9•GDP (G dimer) |
trans loop 1 (atGHSlrp) | α4 (rvhFkqRItad), P-loop (gqSGlgk)†, Switch I (eeRIPKtie)† |
| 5CYP, HsSEPT9•GTPγS (G dimer) |
trans loop 1 (atgHSLrp) | α4 (rvhFkqRItad), P-loop (gqsGlgk)†, Switch I (eeRIPKtie)† |
| 4KV9, SmSEPT10•GDP (G dimer) |
trans loop 1 (ptGHSlks) | α4 (lqkFkaRilse), P-loop (geTGigk)† |
| 4KVA, SmSEPT10•GTP (G dimer) |
trans loop 1 (ptGHSlks) | α4 (lqkFkaRilse), P-loop (geTgigk)† |
| 5AR1, ScCdc11•empty (quasi-G dimer)‡ | trans loop 1 (ptgHGlke) | α4 (lklnkkLimeD) |
*Residues at positions corresponding to ScCdc3 Thr302 were identified based on structure-guided alignments. SEPT2, Arg198; SEPT3, Arg224; SEPT7, Gln210; SEPT9, Arg442; SEPT10, Arg199; Cdc11, Leu187.
†Residues from the other protomer across the G interface.
‡Dimer in solution is mediated by the C-terminal extension, and is unaffected by G interface mutations, thus contacts across the G interface are presumably crystal-induced.