Fig. 2. Fission can be performed throughout the E. coli genome.

(A) Successful Fissions performed. Each colour on the E. coli genome corresponds to ~0.5 Mb. We named the sections A to H. A is dark orange and the other sections are labeled alphabetically in a clockwise sequence. Linker sequence 1, white; oriC black bar; linker sequence 2, gray. Boundaries and homologies of each fission experiment are provided in table S2. Seven fissions are shown, including the 3.43, 0.56 Mb fission (Fig. 1). The 3.45, 0.54 Mb fission (purple Chr. 2) was performed using an E.coli genome in which a ~0.54 Mb section had been recoded (Fig. 3). (B) Growth and luminescence for the generation of the 2.44, 1.55 Mb fission; annotation as in Fig. 1B. Data for other fissions are shown in fig. S4. (C) PCR of clones across new junctions for 2.44 Mb, 1.55 Mb fission. Postfission clones (1 to 5) exhibit products of the expected size, whereas the prefission control does not. Junction PCR for other fissions in fig. S4.