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(A) RT-PCR analysis of regulator genes in macrophages of ALK5 knockdown or AdshSCR induced by OX-LDL; n = 3. (B) Western blot analysis of KLF4 in macrophages of AdshALK5 or AdshSCR induced by OX-LDL; n = 3. (C) Double-immunofluorescence staining of KLF4 (red) in macrophages (CD68, green) transfected with AdshALK5 or AdshSCR upon OX-LDL stimulation; scale bar = 25 μm; n = 5; *P < 0.05 compared with control group.
