Table 2.
Protein-protein interactions between core-clock and light signaling network components.
| Core-Clock Protein | Light Signaling Protein | Experimental Technique | Reference |
|---|---|---|---|
| CCA1 | PhyB | Y2H, Co-IP | (Yeom et al., 2014) |
| FHY3 | Y2H, Co-IP, LCI | (Li et al., 2011a) | |
| DET1 | Y2H, in vitro assay, BiFC, LCI | (Lau et al., 2011) | |
| HY5 | Y2H, in vitro assay | (Andronis et al., 2008) | |
| CSU4 | Y2H, BiFC, Co-IP | (Zhao et al., 2018) | |
| LHY | PhyB | Co-IP | (Yeom et al., 2014) |
| FHY3 | Y2H | (Li et al., 2011a) | |
| DET1 | in vitro assay, LCI | (Lau et al., 2011) | |
| PRR9 PRR7 |
PIF3 | Y2H, in vitro assay, BiFC | (Martín et al., 2018; Zhang et al., 2020) |
| PIF4 | Y2H, in vitro assay | (Martín et al., 2018; Zhang et al., 2020) | |
| PIF7 | in vitro assay | (Zhang et al., 2020) | |
| PRR5 | PIF3 | Y2H, BiFC | (Martín et al., 2018) |
| PIF4 | Y2H, BiFC, Co-IP | (Martín et al., 2018; Zhang et al., 2020; Zhu et al., 2016) | |
| PIF7 | in vitro assay, BiFC, Co-IP | (Zhang et al., 2020) | |
| ZTL | Y2H, in vitro assay, Co-IP, | (Fujiwara et al., 2008; Kiba et al., 2007; Yasuhara et al., 2004) | |
| FKF1 | Y2H, in vitro assay, Co-IP | (Baudry et al., 2010) | |
| LKP2 | Y2H, in vitro assay, Co-IP | (Baudry et al., 2010; Yasuhara et al., 2004) | |
| GI | PhyB | Y2H, Co-IP | (Yeom et al., 2014) |
| PIF1 PIF4 |
Y2H, in vitro assay | (Nohales et al., 2019) | |
| PIF3 PIF5 |
Y2H, in vitro assay, Co-IP | (Nohales et al., 2019) | |
| ZTL | Y2H, in vitro assay, Co-IP | (Kim et al., 2007) | |
| FKF1 | Y2H, in vitro assay, Co-IP | (Sawa et al., 2007) | |
| LKP2 | Y2H, in vitro assay | (Kim et al., 2007) | |
| TOC1 | PhyB | Y2H, Co-IP | (Yeom et al., 2014) |
| PIF1 (PIL5) | Y2H | (Yamashino et al., 2003) | |
| PIF2 (PIL1) | Y2H | (Makino, 2002) | |
| PIF3 | Y2H, BiFC, Co-IP | (Makino, 2002; Soy et al., 2016) | |
| PIF4 | Y2H, Co-IP | (Yamashino et al., 2003; Zhu et al., 2016) | |
| PIF5 (PIL6) | Y2H | (Fujimori et al., 2004; Yamashino et al., 2003) | |
| PIF6 (PIL2) | Y2H | (Yamashino et al., 2003) | |
| PIF7 | 2HP, BiFC | (Kidokoro et al., 2009) | |
| ZTL | Y2H, in vitro assay, Co-IP | (Fujiwara et al., 2008; Mas et al., 2003) | |
| FKF1 | Y2H, in vitro assay, Co-IP | (Baudry et al., 2010; Mas et al., 2003) | |
| LKP2 | Y2H, in vitro assay | (Baudry et al., 2010; Mas et al., 2003; Yasuhara et al., 2004) | |
| LUX | PhyB | Co-IP | (Yeom et al., 2014) |
| PCH1 | AP-MS | (Huang et al., 2015; Huang et al., 2016b) | |
| ELF4 | PCH1 | AP-MS | (Huang et al., 2015; Huang et al., 2016b) |
| ELF3 | PhyB | Y2H, in vitro assay, Co-IP, AP-MS | (Huang et al., 2015; Kim and Somers, 2019; Liu et al., 2001; Yeom et al., 2014) |
| PhyA PhyC PhyD PhyE |
AP-MS | (Huang et al., 2015) | |
| PIF4 | Y2H, BiFC, | (Nieto et al., 2015) | |
| PIF7 | AP-MS | (Huang et al., 2015) | |
| COP1 | Y2H, Co-IP, BiFC, AP-MS | (Huang et al., 2015; Yu et al., 2008) | |
| SPAs (SPA1 to SPA3) | AP-MS | (Huang et al., 2015) | |
| PCH1 | AP-MS | (Huang et al., 2015; Huang et al., 2016b) | |
| ZTL | PhyB | Y2H, in vitro assay | (Jarillo et al., 2001) |
| CRY1 | Y2H, in vitro assay | (Jarillo et al., 2001) | |
| FKF1 | Y2H, Co-IP, FRET | (Takase et al., 2011) | |
| LKP2 | Y2H | (Yasuhara et al., 2004) |
Due to its dual function, ZTL was included as a clock protein as well as a member of the light signaling pathway. AP-MS, Affinity Purification and Mass Spectrometry; BiFC, Bimolecular Fluorescence Complementation; Co-IP, Co-Immunoprecipitation; FRET, Förster Resonance Energy Transfer analysis; LCI, firefly Luciferase Complementation Imaging; Y2H, Yeast Two-Hybrid; 2HP, Two-hybrid system in Protoplasts.