Figure 3:

Mitochondrial superoxide scavenging selectively decreases metastasis in C3H/HeN mitochondrial mice. DMSO vehicle (−) or MitoTEMPO (+) was IP injected into A: 4-week old HH (− n=14 (vehicle only), + n=15 (MitoTEMPO)), and HC (− n=10, + n=15) mice 24 hours and again 1 hour prior to IV injection of K1735-M2 cells and B: 4 week old CC (− n= 9, + n=10), and CH (− n=8, + n=9) mice 24 hours and again 1 hour prior to IV injection of EO771 cells. Mice were euthanized two weeks post cell injection, lungs were harvested, and gross pulmonary metastases were quantified. A: K1735 injected HH vehicle mice had significantly more metastases ($\overline{\mathrm{x}}$ = 94, SEM 10) than HC vehicle ($\overline{\mathrm{x}}$ = 16, SEM 3), HH MitoTEMPO ($\overline{\mathrm{x}}$ = 11, SEM 2), and HC MitoTEMPO ($\overline{\mathrm{x}}$ = 18, SEM 4) treated mice. B: EO771 injected CH vehicle treated mice had more metastases ($\overline{\mathrm{x}}$ = 24), than CC vehicle ($\overline{\mathrm{x}}$ = 8), CC MitoTEMPO ($\overline{\mathrm{x}}$ = 9), and CH MitoTEMPO ($\overline{\mathrm{x}}$ = 11) treated mice.