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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cancer Res. 2019 Nov 5;80(5):1219–1227. doi: 10.1158/0008-5472.CAN-19-0312

Figure 3. Transient loss of LI stem cell radiation resistance upon plating of single cells.

Figure 3.

(A) Dose survival curves of organoids derived from single Lgr5-GFP+ intestinal stem cells that were obtained from symmetrically-dividing stem cell colonies, irradiated at 24h after plating and counted at 6 days post-irradiation. (B) Dose survival curve of organoids derived from single CD44highcKit− intestinal stem cells that had been sorted from C57BL/6 mice irradiated at 24h after plating and counted at 6 days post-irradiation. (C) LI stem cells recover resistance to radiation at 3 days after plating. Single CD44highcKit- intestinal cells were sorted from C57BL/6 mice, irradiated with 8Gy on successive days after plating, and organoids were counted at 6–7 days post-irradiation. Data (mean±standard error) are collated from at least 2 independent experiments (A-C).