Skip to main content
. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cancer Res. 2020 Jan 3;80(5):950–963. doi: 10.1158/0008-5472.CAN-19-3460

Figure 2. Low DRAIC expression is associated with increased NF-κB activity.

Figure 2.

(A) Gene set enrichment analysis revealed that NF-κB targets are upregulated in tumors in the lowest third of DRAIC expression compared to tumors in the highest third of DRAIC expression. (B) The expression of genes involved in TNFα signaling via NF-κB were found to be negatively correlated with DRAIC expression through Guilt by Association analysis. (C) Box-plot shows expression of indicated NF-κB target gene levels in prostate cancers in TCGA in the highest third of DRAIC expression (Red) versus those in the lowest third (Blue). ***P<0.001, **P <0.01, *P<0.05, Wilcoxon rank sum test. (D) DRAIC overexpression in C4–2B cells measured by RT-qPCR and normalized to 18S RNA. (E) NF-κB reporter assay (Firefly luciferase/Renilla luciferase) in PC3M and C4–2B cells with EV or overexpressing FL DRAIC. (F-G) DRAIC levels (F) and NF-κB luciferase reporter assay (G) in LNCaP cells transfected with negative control siRNA (NC) or two different siRNAs (50 nM) against DRAIC. For (D) to (G) results expressed as mean ± s.d, n = 3. The p values were calculated using Student’s t-test, **P<0.01, *P<0.05.