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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Cancer Res. 2020 Jan 7;80(5):1036–1048. doi: 10.1158/0008-5472.CAN-19-0284

Figure 3.

Figure 3.

OB-Runx2 deficiency attracts MM cells via increased cytokines in BM. A-C, Migration assays were conducted with 2×105 5TGM1 MM cells seeded on 8-μm pore inserts and, as a chemoattractant in the bottom of the well, either BMS from OB-Runx2+/+ or OB-Runx2−/− mice (n=3 mice/group) (A); CM obtained from primary OBs harvested from the long bones of OB-Runx2+/+ and OB-Runx2−/− mice and cultured in proliferating medium for 3 days (n=3 mice/group) (B), or BMS from OB-Runx2−/− mice pre-incubated for 30 minutes with neutralizing antibodies against VCAM-1 (10 μg/ml) or OPN (10 μg/ml) or with isotype IgG (control) (n=3 mice/group) (C). The number of MM cells migrated into the bottom of each well was counted after 24 hours. Each sample was analyzed in triplicate and each assay was performed at least two times. Data are presented as mean ± SEM. ***P < 0.001.