Table 1:
Top 20 most differentially expressed (up- and down-regulated) genes on day 6 (LAFC treated) of notochordal differentiation of H9-hESCs as compared to untreated parental control (day 0).
| SN | Gene | Mean Fold Change |
MeanPvalue | (Up/Downregulated |
|---|---|---|---|---|
| 1 | NR2F2 | 203.84 | 4.58E-77 | Upregulated in LAFC |
| 2 | DACH1 | 65.81 | 1.80E-22 | Upregulated in LAFC |
| 3 | DLK1 | 65.03 | 1.04E-230 | Upregulated in LAFC |
| 4 | LIX1 | 52.42 | 8.77E-59 | Upregulated in LAFC |
| 5 | IGFBP5 | 52.16 | 1.08E-49 | Upregulated in LAFC |
| 6 | LRP2 | 52.03 | 1.38E-49 | Upregulated in LAFC |
| 7 | DKK1 | 51.79 | 1.01E-43 | Upregulated in LAFC |
| 8 | PAX6 | 47.52 | 3.58E-65 | Upregulated in LAFC |
| 9 | WLS | 43.53 | 9.74E-71 | Upregulated in LAFC |
| 10 | SP5 | 43.07 | 7.41E-46 | Upregulated in LAFC |
| 11 | MYO3B | 42.74 | 5.31E-16 | Upregulated in LAFC |
| 12 | CNTN2 | 42.52 | 1.32E-91 | Upregulated in LAFC |
| 13 | ZNF503 | 41.44 | 5.98E-31 | Upregulated in LAFC |
| 14 | SOX1 | 38.76 | 1.76E-20 | Upregulated in LAFC |
| 15 | LMX1A | 37.67 | 1.53E-14 | Upregulated in LAFC |
| 16 | SCUBE2 | 27.90 | 8.79E-12 | Upregulated in LAFC |
| 17 | MEIS2 | 27.26 | 6.25E-11 | Upregulated in LAFC |
| 18 | PRTG | 27.12 | 2.65E-216 | Upregulated in LAFC |
| 19 | ILDR2 | 25.22 | 5.03E-11 | Upregulated in LAFC |
| 20 | POU3F2 | 25.04 | 4.77E-14 | Upregulated in LAFC |
| 21 | LINC00678 | 318.23 | 6.98E-107 | Downregulated in LAFC |
| 22 | MT2A | 147.65 | 7.99E-90 | Downregulated in LAFC |
| 23 | MT1G | 132.56 | 3.95E-48 | Downregulated in LAFC |
| 24 | MT1H | 94.17 | 6.23E-36 | Downregulated in LAFC |
| 25 | MT1E | 78.92 | 3.08E-43 | Downregulated in LAFC |
| 26 | NTS | 57.22 | 1.56E-31 | Downregulated in LAFC |
| 27 | LEFTY1 | 47.76 | 1.87E-21 | Downregulated in LAFC |
| 28 | TDGF1 | 44.30 | 1.22E-114 | Downregulated in LAFC |
| 29 | FOXD3.AS1 | 43.36 | 2.73E-66 | Downregulated in LAFC |
| 30 | LINC00458 | 41.53 | 1.53E-20 | Downregulated in LAFC |
| 31 | C9orf135 | 40.50 | 8.15E-33 | Downregulated in LAFC |
| 32 | CXCL5 | 34.55 | 4.98E-32 | Downregulated in LAFC |
| 33 | TMEM30B | 32.20 | 1.11E-16 | Downregulated in LAFC |
| 34 | NANOG | 30.64 | 4.16E-17 | Downregulated in LAFC |
| 35 | LOC101929194 | 29.66 | 2.63E-25 | Downregulated in LAFC |
| 36 | MT1F | 29.05 | 5.83E-16 | Downregulated in LAFC |
| 37 | D21S2088E | 28.16 | 6.74E-27 | Downregulated in LAFC |
| 38 | CHGA | 24.97 | 4.50E-60 | Downregulated in LAFC |
| 39 | LCK | 24.13 | 8.02E-55 | Downregulated in LAFC |
| 40 | NPFFR2 | 21.65 | 2.01E-13 | Downregulated in LAFC |