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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Mol Cancer Res. 2019 Nov 19;18(3):463–476. doi: 10.1158/1541-7786.MCR-19-0217

Figure 2. HSF1 is not required for T cell development and its genetic elimination does not affect the immune phenotype of Pten-loss-driven T-ALLs.

Figure 2.

(A) Representative FACS-based immunophenotypic analysis of T-ALLs from PtenKO or Hsf1- PtenKO mice assayed at an advanced stage of disease. Initial gating was on T-ALL blasts based on their forward and side scatters. Surface markers analyzed on the leukemic blasts. DN, double negative. The percent of cell populations is indicated in the quadrants.

(B) Clonality assessment by genomic TCRβ rearrangement. T-ALL at an advanced stages of disease recovered from PtenKO or Hsf1- PtenKO mice were analyzed by semi-quantitative PCR analysis of the indicated Vβ1-to DJβ1.7 and Vβ2-to-DJβ2.7 rearrangements. DNA prepared from normal WT thymus (Thy) was included in the analysis. GL: germline, Thy: Thymus, M: molecular marker, black arrows: Dβ-Jβ rearrangements.