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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Mol Cancer Res. 2019 Nov 19;18(3):463–476. doi: 10.1158/1541-7786.MCR-19-0217

Figure 4. Suppression of mTORC1 signaling following Hsf1 depletion in human T-ALL cell lines.

Figure 4.

(A) Representative WBs showing MAPK/ERK and PI3K/AKT/mTOR signaling protein expression in human T-ALL cell lines (CCL119 and CUTLL1) following silencing of Hsf1 by doxycycline-inducible shRNA expression for the indicated times. Cells expressing scramble shRNA were used as control (n=3 experiments).

(B) Cell cycle analysis of CCL119 and CUTLL1 cell lines following silencing of Hsf1 as described in panel A. Cells were resuspended in propidium Iodide (PI) solution (100μg/ml ribonuclease A, 50μg/ml PI in PBS) and incubated for 30 min at room temperature and analyzed by FACS. The percentage of cells in G1, S, and G2/M cell-cycle phases are indicated (n=3 independent analyses).