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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2019 Dec 17;19(3):731–741. doi: 10.1158/1535-7163.MCT-19-0809

Figure 2. DDR and Roc increase caspase and PARP cleavage and elevate the levels of γH2A.X while decreasing AKT and ERK expression in MPNST cells.

Figure 2.

(A) Protein lysates prepared from STS26T cells treated for 3 days with 1- or 2-IC50 of DDR or Roc were analyzed by Western blots for full-length and cleaved caspase-3/7 and PARP, as well as AKT and ERK1/2. GAPDH served as a loading control. (B) Protein lysates from STS26T cells treated for 1 and 2 days with 1- or 2-IC50 of DDR were probed for the expression of phosphorylated H2A.X (γH2A.X). As a positive control, lysates from HMS-97 human malignant schwannoma cells irradiated with 4 Grays (Gy) of X-ray were used.