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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Clin Cancer Res. 2019 Dec 18;26(5):1094–1104. doi: 10.1158/1078-0432.CCR-19-0909

Figure 2: Proliferation and invasiveness of orthotopic PDX.

Figure 2:

(A) Distribution of Ki67 labeling in PDX derived from primary and recurrent tumors. Ki67 labeling index was determined from tissue microarray cores from 58 orthotopic PDX lines. A mean of 4.5 cores was analyzed from one (n=4) or two (n=54) independent orthotopic tumors for each line. (B) Inverse correlation of Ki67 labeling with time to moribund following intracranial injection of tumor cells. (C) Representative H&E and Ki67 staining for PDX with low, moderate, and high Ki67 labeling indexes. (D) Tumor cell invasion was assessed by immunofluorescence using human specific antibodies to Lamin A/C. Representative sections showing invasion pattern scoring: 1 = unilateral, well-demarcated tumor; 2 = unilateral with infiltrating border; 3A = bilateral, restricted to contralateral midline structures; 3B = bilateral with clear involvement of both hemispheres; 4 = diffuse infiltration bilaterally. (E) Distribution of invasion patterns for 55 orthotopic PDX.