Table 1.
Kinetics parameters of CRY photooligomerization.
| Parameters | Experiment #1 | Experiment #2 | Sources | |
|---|---|---|---|---|
| CRY2 | 30O50 (minute) | 0.83 | 0.78 | HEK 293 |
| 100O50 (minute) | 0.14* | 0.25* | HEK 293 | |
| 10F50 (μmolm−2s−1) | 27.91* | 28.9 | HEK 293 | |
| 22T½ (minute) | 16.58* | 18.62* | HEK 293 | |
| 37T½ (minute) | 5.42 | HEK 293 | ||
| 30O50 (minute) | 0.17* | 0.26 | Arabidopsis | |
| 10F50 (μmolm−2s−1) | 16.89 | 13.56 | Arabidopsis | |
| 10T½ (minute) | 8.42* | Arabidopsis | ||
| 22T½ (minute) | 6.98* | Arabidopsis | ||
| 37T½ (minute) | 5.46* | Arabidopsis | ||
| CRY1 | 30O50 (minute) | 1.47 | 1.70 | Arabidopsis |
| 100O50 (minute) | 0.37* | 0.38 | Arabidopsis | |
| 60F50 (μmolm−2s−1) | 14.90 | 16.21 | Arabidopsis | |
| 22T½ (minute) | 2.56 | 1.82 | Arabidopsis |
The table summarizes results of the experiments for the measurement of kinetics parameters of Arabidopsis CRY1 and CRY2 photooligomerization in HEK293 (Human Embryonic Kidney) cells or Arabidopsis seedlings. Symbols of parameters are the following: 30O50 or 100O50: time (minute) required to reach 50% saturation of CRY photooligomerization at the fluence rate of 30 or 100 μmolm−2s−1, respectively; 10F50 or 60F50: the fluence rate of blue light (μmolm−2s−1) required to achieve 50% CRY photooligomerization after light exposure for 10 or 60 seconds, respectively; 10T1/2, 22T1/2, or 37T1/2: time (minute) required to reach 50% saturation of CRY monomerization at the temperature of 10 °C, 22 °C, or 37 °C, respectively. Numbers with asterisk (*) indicates the measurements were means of three technical repeats, and numbers without asterisk indicates the measurements were derived from one technical repeat.