Figure 6.

Lower doses of Cl-OCH₃ inhibit mammary growth and metastasis in a syngeneic mouse model. Balb/c mice bearing 4T1-Luc cells (orthotopic) were treated with Cl-OCH₃ (2.5 or 7.5 mg/kg/day) by ip injection and effects on tumor volumes (A) body weights (B) and tumor weights (C) were determined. D. Luciferase activities were also determined in mammary tumors and also in spleen, brain, liver, kidney and lungs of the control and treated animals. Results are expressed as means ± SD and significant (p<0.05) treatment – related responses compared to the control group are indicated (*). Average tumor weights in control, 2.5 and 7.5 mg/k/d treated mice were 1.57, 0.91 and 0.47 g and tumor volumes were 579, 156 and 108 mm³ respectively.