Figure 1. Schematic representation of the study design with number of animals used for each experimental set.

In stroke outcome experiments exclusion criteria included insufficient cortical blood flow decline (≥70%) and recovery (≥50%) during occlusion and reperfusion respectively, subarachnoid hemorrhage upon euthanasia, seizures after stroke, and death during surgery or before behavioral evaluation. Due to these criteria 14 mice were eliminated from the planned experiments (3 vehicle-injected, 4 Agaricoglyceride A-injected, 5 no treatment, and 2 AAV vector-injected).