Table 2.
Antibodies used for dendritic cell characterization
| Marker | Role |
|---|---|
| CD11bi | BD Biosciences Cat# 562287, RRID:AB_11154216 |
| CD11c1 | 1:100, BD Biosciences Cat# 558079, RRID:AB_647251 |
| 1:100, Thermo Fisher Scientific Cat# 45–0114-82, RRID:AB_925727 | |
| CD45RAii | 1:800, BD Biosciences Cat# 553380, RRID:AB_394822 |
| CD54iii | BioLegend Cat# 116120, RRID:AB_10612936 |
| CD86iv | Thermo Fisher Scientific Cat# 17–0862-82, RRID:AB_469419 |
| BD Biosciences Cat# 553692, RRID:AB_394994 | |
| CD804 | Thermo Fisher Scientific Cat# 11–0801-86, RRID:AB_465135APC: |
| Biolegend 104714 | |
| IABv | BD Biosciences Cat# 553551, RRID:AB_394918 |
| 1:400, BD Biosciences Cat# 553552, RRID:AB_394919 | |
| Qa2vi | Thermo Fisher Scientific Cat# 11–5996-85, RRID:AB_465353 |
Identifies dendritic cell subtypes
Diao, J., Winter, E., Chen, W., Cantin, C., & Cattral, M. S. (2004). Characterization of distinct conventional and plasmacytoid dendritic cell-committed precursors in murine bone marrow. J Immunol, 173(3), 1826–1833.
Liu, Y. J. (2001). Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity. Cell, 106(3), 259–262.
Identifies naïve dendritic cells.
Naik, S. H., Sathe, P., Park, H. Y., Metcalf, D., Proietto, A. I., Dakic, A., … Shortman, K. (2007). Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat Immunol, 8(11), 1217–1226. doi: 10.1038/ni1522
Regulates antigen uptake.
Sheikh, N. A., & Jones, L. A. (2008). CD54 is a surrogate marker of antigen presenting cell activation. Cancer Immunol Immunother, 57(9), 1381–1390. doi: 10.1007/s00262-008-0474-9
Regulates adaptive immunity.
Fujii, S., Liu, K., Smith, C., Bonito, A. J., & Steinman, R. M. (2004). The linkage of innate to adaptive immunity via maturing dendritic cells in vivo requires CD40 ligation in addition to antigen presentation and CD80/86 costimulation. J Exp Med, 199(12), 1607–1618. doi: 10.1084/jem.20040317
Regulates antigen presentation.
Wilson, N. S., El-Sukkari, D., & Villadangos, J. A. (2004). Dendritic cells constitutively present self antigens in their immature state in vivo and regulate antigen presentation by controlling the rates of MHC class II synthesis and endocytosis. Blood, 103(6), 2187–2195. doi: 10.1182/blood-2003-08-2729
Regulates alternative antigen processing.
Oliveira, Cláudia C., van Veelen, Peter A., Querido, Bianca, de Ru, Arnoud, Sluijter, Marjolein, Laban, Sandra, … van Hall, Thorbald. (2010). The nonpolymorphic MHC Qa-1<sup>b</sup> mediates CD8<sup>+</sup> T cell surveillance of antigen-processing defects. The Journal of Experimental Medicine, 207(1), 207–221. doi: 10.1084/jem.20091429
Shang, Shaobin, Siddiqui, Sarah, Bian, Yao, Zhao, Jie, & Wang, Chyung-Ru. (2016). Nonclassical MHC Ib-restricted CD8+ T Cells Recognize Mycobacterium tuberculosis-Derived Protein Antigens and Contribute to Protection Against Infection. PLOS Pathogens, 12(6), e1005688. doi: 10.1371/journal.ppat.1005688