Figure 1.

lncRNA CEBPA-AS1 and NCOR2 Are Poorly Expressed in OS

(A) The heatmap of the top 10 genes that are differentially expressed in OS versus GEO: GSM1017466, GSM1017467, and GSM1017468 are normal samples, whereas GEO: GSM1017514, GSM1017515, and GSM1017516 are OS samples. (B) qRT-PCR analysis of lncRNA CEBPA-AS1 expression, and the mRNA expression of NCOR2 in normal tissues and OS tissues. (C) Western blot analysis of NCOR2 protein expression in normal tissues and OS tissues. The band intensity is assessed. (D) qRT-PCR analysis of lncRNA CEBPA-AS1 expression and the mRNA expression of NCOR2 in four OS cell lines (MG63, HOS, G292, and SAOS-2). *p < 0.05 versus the normal tissues. The measurement data are expressed as the mean ± SD. (B and C) Comparisons between two groups are analyzed using non-paired t tests. (D) Comparisons among multiple groups are analyzed using one-way ANOVA. n = 36.